Three-dimensional silk fibroin microsphere-nanofiber scaffolds for vascular tissue engineering

Medicine in Novel Technology and Devices

Guoliang Ying¹  Ali K. Yetisen²  Xiaoying Xie³  Qiang Liu⁴  Haifeng Liu⁴  Danyu Yao⁵  Yubo Fan⁶  Nan Jiang⁷ 

[1] Department of Chemistry, Stanford University, CA, 94305, USA;Division of Engineering in Medicine, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, MA, 02139, USA;Department of Chemical Engineering, Imperial College London, London, SW7 2AZ, UK;Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beijing Advanced Innovation Centre for Biomedical Engineering, Beihang University, Beijing, 100191, People’s Republic of China;School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, 02138, USA;School of Life Information Science and Instrument Engineering, Hangzhou Dianzi University, Hangzhou, 310018, Zhejiang Province, People’s Republic of China;School of Materials Science and Engineering, Wuhan Institute of Technology, Wuhan, 430205, People’s Republic of China;

关键词: Microfluidics;    Silk fibroin;    Electrospinning;    Micro/nanostructure;    Tissue engineering;   

DOI  :  

来源: DOAJ

【 摘 要 】

A significant limitation in the engineering of artificial small-diameter vascular scaffolds is that the number of endothelial cells (ECs) is not sufficient to generate a confluent coverage of the vascular scaffolds, so that the surfaces of vascular scaffolds form thrombus via platelet adhesion and aggregation. Thrombus decrease relies on three-dimensional (3D) scaffolds to mimic the natural extracellular matrix (ECM) as templates to regulate cell behavior and facilitate tissue maturation. Here, we developed 3D scaffolds consisting of silk fibroin (SF) nanofibers and homogeneous microspheres by electrospinning and microfluidics. The nanofibers with diameters ranging from 250 to 350 ​nm doped with microspheres (2–10 ​μm) formed bridge-shaped structures. ECs were seeded and maintained on the 3D microsphere-nanofiber scaffolds with a mean fiber diameter of 300 ​nm. A 10% higher ratio of cell proliferation on 3D microsphere-nanofiber SF scaffolds was noted as compared to that on microporous and sponge-like SF scaffolds with small surface network fabricated by freeze-drying. Moreover, the gene transcript levels including CD146, VE-C and PECAM-1 were better preserved on 3D microsphere-nanofiber SF scaffolds than those on freeze-dried scaffolds. Thus, the developed 3D microsphere-nanofiber structure may have a myriad of applications in vascular tissue engineering scaffolds and cardiovascular devices.

【 授权许可】

Unknown