Isolation of spheroid-forming single cells from gastric cancer cell lines: enrichment of cancer stem-like cells

BioTechniques

Yeul Hong Kim¹  Jong Won Lee¹  Young Soo Park²  Jae Sook Sung²  Seok Chung³ 

[1] 1Brain Korea 21 Plus Project for Biomedical Science, Korea University College of Medicine, Seoul 02841, Republic of Korea;2Cancer Research Institute, Korea University, Seoul 02841, Republic of Korea;4School of Mechanical Engineering, College of Engineering, Korea University, Seoul 02841, Republic of Korea;

关键词: cancer stem cells;    gastric cancer;    spheroids;   

DOI  :  10.2144/btn-2018-0046

来源: DOAJ

【 摘 要 】

Isolation of spheroid-forming cells is important to investigate cancer stem cell (CSC) characteristics. However, conventional tumor spheroid culture methods have not proven suitable because the aggregated spheroids generally maintain their original heterogeneity and harbor multiple cells with various characteristics. Here we cultured spheroids using a polydimethylsiloxane microwell-based method and a limiting dilution protocol. We then isolated and enriched for CSCs that formed single cell-derived spheroids from gastric cancer cell lines. Cells from the microwell demonstrated higher self-renewal, increased expression of stem cell markers and resistance to apoptosis compared with spheroid cells made by the traditional method. This novel approach allows efficient cancer stem cell isolation and represents a step forward in cancer stem cell studies.

【 授权许可】

Unknown