Oenanthe Javanica Extract Protects Mouse Skin from UVB Radiation via Attenuating Collagen Disruption and Inflammation

International Journal of Molecular Sciences

Hyunjung Kim¹  Jong-Dai Kim²  KiSeob Kim³  DaeWon Kim⁴  JoonHa Park⁵  Young Her⁶  JiHyeon Ahn⁷  YunLyul Lee⁸  Bich-Na Shin⁸  Moo-Ho Won⁹  Minah Song^1,10 

[1] Bio Inc., Chuncheon, Gangwon 24232, Korea;Center for Virus Research and Testing, Korea Research Institute of Chemical Technology, Daejeon 34114, Korea;;Da Rum &Department of Biochemistry and Molecular Biology, and Research Institute of Oral Sciences, College of Dentistry, Gangnung-Wonju National University, Gangneung, Gangwon 25457, Korea;Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon, Gangwon 24252, Korea;Department of Dermatology, Kangwon National University Hospital, Kangwon National University School of Medicine, Chuncheon, Gangwon 24289, Korea;Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, Gangwon 24341, Korea;Department of Physiology, College of Medicine, Hallym University, Chuncheon, Gangwon 24252, Korea;Division of Food Biotechnology, School of Biotechnology, Kangwon National University, Chuncheon, Gangwon 24341, Korea;Knotus Co. Ltd., Incheon 406-840, Korea;

关键词: anti-inflammation;    Oenanthe javanica extract;    photoprotection;    skin;    ultraviolet B;   

DOI  :  10.3390/ijms20061435

来源: DOAJ

【 摘 要 】

In recent years, the use of botanical agents to prevent skin damage from solar ultraviolet (UV) irradiation has received considerable attention. Oenanthe javanica is known to exert anti-inflammatory and antioxidant activities. This study investigated photoprotective properties of an Oenanthe javanica extract (OJE) against UVB-induced skin damage in ICR mice. The extent of skin damage was evaluated in three groups: control mice with no UVB, UVB-exposed mice treated with vehicle (saline), and UVB-exposed mice treated with 1% extract. Photoprotective properties were assessed in the dorsal skin using hematoxylin and eosin staining, Masson trichrome staining, immunohistochemical staining, quantitative real-time polymerase chain reaction, and western blotting to analyze the epidermal thickness, collagen expression, and mRNA and protein levels of type I collagen, type III collagen, and interstitial collagenases, including matrix metalloproteinase (MMP)-1 and MMP-3. In addition, tumor necrosis factor (TNF)-α and cyclooxygenase (COX)-2 protein levels were also assessed. In the UVB-exposed mice treated with extract, UV-induced epidermal damage was significantly ameliorated. In this group, productions of collagen types I and III were increased, and expressions of MMP-1 and MMP-3 were decreased. In addition, TNF-α and COX-2 expressions were reduced. Based on these findings, we conclude that OJE displays photoprotective effects against UVB-induced collagen disruption and inflammation and suggest that Oenanthe javanica can be used as a natural product for the treatment of photodamaged skin.

【 授权许可】

Unknown