Identification of Breast Cancer Stem Cell Related Genes Using Functional Cellular Assays Combined With Single-Cell RNA Sequencing in MDA-MB-231 Cells

Frontiers in Genetics

Thomas Kroneis¹  Göran Landberg³  Joakim Karlsson⁴  Erik Larsson⁴  Emma Persson⁵  Salim Ghannoum⁵  Emma Jonasson⁵  Anders Ståhlberg⁶ 

[1] Department of Cell Biology, Histology and Embryology, Gottfried Schatz Research Center, Medical University of Graz, Graz, Austria;Department of Clinical Genetics and Genomics, Sahlgrenska University Hospital, Gothenburg, Sweden;Department of Clinical Pathology, Sahlgrenska University Hospital, Gothenburg, Sweden;Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden;Department of Pathology and Genetics, Institute of Biomedicine, Sahlgrenska Cancer Center, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden;Wallenberg Centre for Molecular and Translational Medicine, University of Gothenburg, Gothenburg, Sweden;

关键词: breast cancer;    cancer stem cell;    cell proliferation assay;    mammosphere assay;    single-cell analysis;    single-cell RNA sequencing;   

DOI  :  10.3389/fgene.2019.00500

来源: DOAJ

【 摘 要 】

Breast cancer tumors display different cellular phenotypes. A growing body of evidence points toward a population of cancer stem cells (CSCs) that is important for metastasis and treatment resistance, although the characteristics of these cells are incomplete. We used mammosphere formation assay and label-retention assay as functional cellular approaches to enrich for cells with different degree of CSC properties in the breast cancer cell line MDA-MB-231 and performed single-cell RNA sequencing. We clustered the cells based on their gene expression profiles and identified three subpopulations, including a CSC-like population. The cell clustering into these subpopulations overlapped with the cellular enrichment approach applied. To molecularly define these groups, we identified genes differentially expressed between the three subpopulations which could be matched to enriched gene sets. We also investigated the transition process from CSC-like cells into more differentiated cell states. In the CSC population we found 14 significantly upregulated genes. Some of these potential breast CSC markers are associated to reported stem cell properties and clinical survival data, but further experimental validation is needed to confirm their cellular functions. Detailed characterization of CSCs improve our understanding of mechanisms for tumor progression and contribute to the identification of new treatment targets.

【 授权许可】

Unknown