Clinical helminthiases in Thailand border regions show elevated prevalence levels using qPCR diagnostics combined with traditional microscopic methods

Parasites & Vectors

Nirundorn Homsuwan¹  Tippayarat Yoonuan¹  Poom Adisakwattana¹  Akkarin Poodeepiyasawat¹  Orawan Phuphisut¹  Donald P. McManus²  Catherine A. Gordon²  Geoffrey N. Gobert³  Louise E. Atkinson³  Angela Mousley³ 

[1] Department of Helminthology, Faculty of Tropical Medicine, Mahidol University;Molecular Parasitology Laboratory, Infectious Diseases Division, QIMR Berghofer Medical Research Institute;School of Biological Sciences, Queenʼs University Belfast;

关键词: Helminthiases;    Kato-Katz;    molecular diagnostics;    qPCR;    Thailand border regions;    Southeast Asia;   

DOI  :  10.1186/s13071-020-04290-0

来源: DOAJ

【 摘 要 】

Abstract Background Under-regulated national borders in Southeast Asia represent potential regions for enhanced parasitic helminth transmission and present barriers to helminthiasis disease control. Methods Three Thailand border regions close to Myanmar, Laos and Cambodia were surveyed for clinical parasitic helminth disease. In-field microscopy was performed on stools from 567 individuals. Sub-samples were transported to Bangkok for molecular analysis comprising three multiplex qPCR assays. Results The overall helminth infection prevalence was 17.99% as assessed by Kato-Katz and 24.51% by qPCR. The combined prevalence of the two methods was 28.57%; the most predominant species detected were Opisthorchis viverrini (18.34%), hookworm (6.88%; Ancylostoma spp. and Necator americanus), Ascaris lumbricoides (2.29%) and Trichuris trichiura (1.76%). Conclusions These data demonstrate the value of molecular diagnostics for determining more precise prevalence levels of helminthiases in Southeast Asia. Availability of such accurate prevalence information will help guide future public health initiatives and highlights the need for more rigorous surveillance and timely intervention in these regions.

【 授权许可】

Unknown