Plant Regeneration and In Vitro Growth Performance of Male-Sterile Somatic Plantlets of Sugi (Japanese Cedar, Cryptomeria japonica) Derived from Different Embryogenic Cell Lines

Forests

Momi Tsuruta¹  Saneyoshi Ueno¹  Tsuyoshi E. Maruyama¹  Takumi Kaneeda²  Yoshinari Moriguchi² 

[1] Department of Forest Molecular Genetics and Biotechnology, Forestry and Forest Products Research Institute, Matsunosato 1, Tsukuba 305-8687, Japan;Graduate School of Science and Technology, Niigata University, Ikarashi 8050, Niigata 950-2181, Japan;

关键词: Cuppressaceae;    plantlet growth;    propagation;    somatic embryogenesis;    sugi pollinosis;    tissue culture;   

DOI  :  10.3390/f12111592

来源: DOAJ

【 摘 要 】

With the spread of pollinosis caused by sugi (Japanese cedar, Cryptomeria japonica) pollen, the use of pollen-free somatic seedlings of sugi is expected in Japan. However, there is a lack of knowledge on the relationship between the abilities during somatic embryogenesis, initial in vitro growth traits, and subsequent growth of somatic seedlings. In the present study, we provide the first basic information on somatic embryo maturation efficiency, somatic embryo germination, and plantlet conversion frequencies, as well as on in vitro growth performance of pollen-free somatic plantlets derived from different embryogenic cell lines (ECLs). Somatic embryo maturation efficiency varied from 34 to 514 cotyledonary embryos per plate and the average for the 19 ECLs tested was 244 embryos per plate. Subsequently, the overall average rates of somatic embryo germination and conversion among ECLs were 87.8% and 85.3%, respectively. The results of in vitro growth performance of pollen-free somatic plantlets showed significant differences in growth rate among ECLs.

【 授权许可】

Unknown