Effects of LED light on Acacia melanoxylon bud proliferation in vitro and root growth ex vitro

Open Life Sciences

Ding Guochang¹  Wu Sipan²  Liu Li²  Lin Sizu²  Huang Meng²  Li Shubin²  Zhou Lili³ 

[1] College of Arts College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, 350002, P. R. China;Forestry College, Fujian Agriculture and Forestry University, Fuzhou, 350002, P. R. China;Institute of Oceanography, Minjiang University, Fuzhou, 350108, P. R. China;

关键词: acacia melanoxylon;    light-emitting diode (led) light;    multiple bud proliferation;    plantlet growth;    root growth;   

DOI  :  10.1515/biol-2019-0039

来源: DOAJ

【 摘 要 】

This study examines the effects of light emitting diodes (LEDs) on tissue culture proliferation of Acacia melanoxylon plantlets among five different clones (FM1, FM2, FM4, FM5, and FM10). Shoot bud apex cuttings were transplanted onto Murashige and Skoog basal medium containing 0.1 mg L-1 6-benzyladenine and 0.5 mg L-1 naphthalene acetic acid and cultured in vitro for 40 days. Root growth was studied under different light intensities and photoperiods ex vitro. The bud proliferation coefficient was greatest under a light intensity of 45 μmol m-2 s-1 photosynthetic photon flux and photoperiod of 16 h light, but decreased as the light intensity increased. However, the greatest light intensity was beneficial for the growth of robust plantlets. Plantlets exposed to red and blue LED combinations grew tall and green, with a small number of roots. Plantlets also grew taller and some roots expanded under the longer photoperiod. Increased light intensity had positive effects on root number and rooting rate, and prolonged light greatly increased root number. Therefore, lower light intensity and a short photoperiod were beneficial for bud proliferation, while red/blue LED combinations, increased light intensity, and longer light illumination were beneficial for plantlet growth and root growth of Acacia melanoxylon.

【 授权许可】

Unknown