Isolation of human bone marrow stromal cells from bone marrow biopsies for single-cell RNA sequencing

STAR Protocols

Nils B. Leimkühler¹  Inge A.M. Snoeren²  Stijn N.R. Fuchs³  Rebekka K. Schneider⁴  Hélène F.E. Gleitz⁴ 

[1] Corresponding author;Current address: Department of Developmental Biology, Erasmus MC, Rotterdam, 3015GD, the Netherlands;Oncode Institute, Erasmus MC, Rotterdam, 3015GD, the Netherlands;Department of Hematology, Erasmus MC, Rotterdam, 3015GD, the Netherlands;

关键词: Cell Biology;    Cell isolation;    Flow Cytometry/Mass Cytometry;    Cancer;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Bone marrow (BM) mesenchymal stromal cells play an important role in regulating stem cell quiescence and homeostasis; they are also key contributors to various hematological malignancies. However, human bone marrow stromal cells are difficult to isolate and prone to damage during isolation. This protocol describes a combination of mechanical and enzymatic isolation of BM stromal cells from human BM biopsies, followed by FACS sorting to separate stromal sub-populations including mesenchymal stromal cells, fibroblasts, and Schwann cells for single-cell RNA sequencing.For complete details on the use and execution of this protocol, please refer to Leimkühler et al. (2020).

【 授权许可】

Unknown