Changes in Exosomal miRNA Composition in Thyroid Cancer Cells after Prolonged Exposure to Real Microgravity in Space

International Journal of Molecular Sciences

Thomas J. Corydon¹  Manfred Infanger²  Marcus Krüger²  Markus Wehland²  Daniela Grimm²  Stefan Riwaldt²  Markus Braun³  Paolo Neviani⁴  Petra M. Wise⁴ 

[1] Department of Biomedicine, Aarhus University, Ole Worms Allé 4, 8000 Aarhus C, Denmark;Department of Microgravity and Translational Regenerative Medicine, Clinic for Plastic, Aesthetic and Hand Surgery, Otto von Guericke University, Universitätsplatz 2, 39106 Magdeburg, Germany;Deutsches Zentrum für Luft- und Raumfahrt (DLR), Raumfahrtagentur im DLR, Bonn-Oberkassel, 53227 Bonn, Germany;The Saban Research Institute, Children’s Hospital Los Angeles, University of Southern California, 4650 Sunset Blvd., Los Angeles, CA 90027, USA;

关键词: thyroid cancer;    cell culture;    exosomes;    microgravity;    spaceflight;    miRNA;   

DOI  :  10.3390/ijms222312841

来源: DOAJ

【 摘 要 】

As much as space travel and exploration have been a goal since humankind looked up to the stars, the challenges coming with it are manifold and difficult to overcome. Therefore, researching the changes the human organism undergoes following exposure to weightlessness, on a cellular or a physiological level, is imperative to reach the goal of exploring space and new planets. Building on the results of our CellBox-1 experiment, where thyroid cancer cells were flown to the International Space Station, we are now taking advantage of the newest technological opportunities to gain more insight into the changes in cell–cell communication of these cells. Analyzing the exosomal microRNA composition after several days of microgravity might elucidate some of the proteomic changes we have reported earlier. An array scan of a total of 754 miRNA targets revealed more than 100 differentially expressed miRNAs in our samples, many of which have been implicated in thyroid disease in other studies.

【 授权许可】

Unknown