Changes in Exosome Release in Thyroid Cancer Cells after Prolonged Exposure to Real Microgravity in Space

International Journal of Molecular Sciences

ThomasJuhl Corydon¹  Manfred Infanger²  Marcus Krüger²  Markus Wehland²  Daniela Grimm²  Stefan Riwaldt²  Markus Braun³  Paolo Neviani⁴  PetraM. Wise⁴ 

[1] Department of Biomedicine, Aarhus University, Ole Worms Allé 4, 8000 Aarhus C, Denmark;Department of Microgravity and Translational Regenerative Medicine, Clinic for Plastic, Aesthetic and Hand Surgery, Otto von Guericke University, Universitätsplatz 2, 39106 Magdeburg, Germany;Deutsches Zentrum für Luft- und Raumfahrt (DLR), Raumfahrtmanagement Bonn-Oberkassel, 53227 Bonn, Germany;The Saban Research Institute, Children’s Hospital Los Angeles, University of Southern California, 4650 Sunset Blvd, Los Angeles, CA 90027, USA;

关键词: thyroid cancer;    cell culture;    exosomes;    microgravity;    spaceflight;    transmembrane proteins;   

DOI  :  10.3390/ijms22042132

来源: DOAJ

【 摘 要 】

Space travel has always been the man’s ultimate destination. With the ability of spaceflight though, came the realization that exposure to microgravity has lasting effects on the human body. To counteract these, many studies were and are undertaken, on multiple levels. Changes in cell growth, gene, and protein expression have been described in different models on Earth and in space. Extracellular vesicles, and in particular exosomes, are important cell-cell communicators, being secreted from almost all the cells and therefore, are a perfect target to further investigate the underlying reasons of the organism’s adaptations to microgravity. Here, we studied supernatants harvested from the CellBox-1 experiment, which featured human thyroid cancer cells flown to the International Space Station during the SpaceX CRS-3 cargo mission. The initial results show differences in the number of secreted exosomes, as well as in the distribution of subpopulations in regards to their surface protein expression. Notably, alteration of their population regarding the tetraspanin surface expression was observed. This is a promising step into a new area of microgravity research and will potentially lead to the discovery of new biomarkers and pathways of cellular cross-talk.

【 授权许可】

Unknown