Dictyostelium Cell Fixation: Two Simple Tricks

Methods and Protocols

Ralph Gräf¹  Michael Koonce²  Irina Tikhonenko² 

[1] Department of Cell Biology, University of Potsdam, Potsdam-Golm 14476, Germany;Division of Translational Medicine, Wadsworth Center, NYS Department of Health, Albany, NY 12237, USA;

关键词: Dictyostelium;    cell fixation;    microscopy;    microtubule;    cytoskeleton;   

DOI  :  10.3390/mps3030047

来源: DOAJ

【 摘 要 】

We share two simple modifications to enhance the fixation and imaging of relatively small, motile, and rounded model cells. These include cell centrifugation and the addition of trace amounts of glutaraldehyde to existing fixation methods. Though they need to be carefully considered in each context, they have been useful to our studies of the spatial relationships of the microtubule cytoskeletal system.

【 授权许可】

Unknown