Immunohistochemical Study and Tissue Array Method for Comparison Between Expression of Tumor Suppressor Genes and Intercellular Adhesive Molecules In Colorectal Adenocarcinoma and Nontumoral Colon

Cell Journal

关键词: Colorectal adenocarcinoma;    Immunohistochemistry;    Tissue array;    P53;    P16;    P21;    E-cadherin;    beta catenin;   

DOI  :  

来源: DOAJ

【 摘 要 】

Introduction: Colorectal Carcinoma is a main health problem in manycountries and the third common cancer in Iran. This malignancy atpresent is the most curable carcinoma of gastrointestinal tract. Variationin the expression of the proteins produced by P53, P21, P16, E-cadherin,and β-catenin genes have been noted in this malignancy and may beimportant in the prognosis and therapeutic response rate. The aim of thisstudy was to compare the frequency and pattern of expression of theseproteins in tumoral and nontumoral colonic mucosa. The correlation withprognostic factors including tumor stage, grade, and vascular andperineural invasion was also determined.Material and Methods: The paraffin blocks from tumoral andnontumoral parts of the colon obtained from 58 patients with colorectaladenocarcinoma were studied along with 50 colectomic cases inindividuals without malignancy. Cylindrical tissue fragments wereobtained from appropriate parts of donor blocks by using a 2.5 mmpunch biopsy instrument. Each 30 samples were manually arrayed inone tissue array block. Expression of above genes was investigatedafter sectioning the blocks and immunohistochemical staining of slides.Results: The expression of P53 in tumor cells was significantly morecommon than in colonic nontumor cells and colon of individuals withouttumor (p<0.001); expression of this protein in tumoral tissues wasdirectly related to vascular invasion (p=0.017). The expressionfrequency of P21 and P16 in tumor cells was less than nontumoral tissuesof patients with cancer and patients without cancer (p<0.001). These twogene products showed no correlation with prognostic factors. Theexpression frequency of membranous E-cadherin and β-catenin in tumorcells was not different from controls, while the membranous expressionof E-cadherin was inversely related to cell differentiation (p=0.023) andvascular invasion (p=0.025). In addition, the membranous expression ofβ-catenin was inversely related to vascular invasion (p=0.049).Cytoplasmic and nuclear expression of β-catenin in tumor cells weresignificantly higher than their expression in the controls (p<0.001).Cytoplasmic expression of this marker was inversely related to diseasestage (p=0.013), while its nuclear expression was inversely related tocell differentiation (p=0.012).Conclusion: According to our data, it seems that we are able to predictaggressive capacity of the colorectal tumor by determining the frequencyand pattern of expression of P53, E-cadherin and β-catenin proteins.These studies can be done simply on formalin-fixed small biopsysamples before surgery to provide valuable information for surgeons,gastroenterologists, and oncologists to choose the best therapeuticapproach and predict the therapeutic response. Manual tissue arraymethod is believed to be an economical technique for similar researchprojects.

【 授权许可】

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