A Comparison of the Capacity of Mesenchymal Stromal Cells for Cartilage Regeneration Depending on Collagen-Based Injectable Biomimetic Scaffold Type

Life

Alexandra D. Kirillova¹  Victor I. Sevastianov¹  Yulia B. Basok¹  Evgeniy A. Nemets¹  Sergey V. Gautier¹  Alexey M. Grigoriev¹  Ludmila A. Kirsanova¹  Anastasia M. Subbot² 

[1] Department for Biomedical Technologies and Tissue Engineering, The Shumakov National Medical Research Center of Transplantology and Artificial Organs, 1 Shchukinskaja St., 123182 Moscow, Russia;Laboratory of Fundamental Research in Ophtalmology, The Research Institute of Eye Diseases, 11A, B Rossolimo St., 119021 Moscow, Russia;

关键词: mesenchymal stromal cells;    articular cartilage;    osteoarthrosis;    collagen;    hydrogel;    decellularization;   

DOI  :  10.3390/life11080756

来源: DOAJ

【 摘 要 】

Mesenchymal stromal cells (MSCs) have shown a high potential for cartilage repair. Collagen-based scaffolds are used to deliver and retain cells at the site of cartilage damage. The aim of the work was a comparative analysis of the capacity of the MSCs from human adipose tissue to differentiate into chondrocytes in vitro and to stimulate the regeneration of articular cartilage in an experimental model of rabbit knee osteoarthrosis when cultured on microheterogenic collagen-based hydrogel (MCH) and the microparticles of decellularized porcine articular cartilage (DPC). The morphology of samples was evaluated using scanning electron microscopy and histological staining methods. On the surface of the DPC, the cells were distributed more uniformly than on the MCH surface. On day 28, the cells cultured on the DPC produced glycosaminoglycans more intensely compared to the MCH with the synthesis of collagen type II. However, in the experimental model of osteoarthrosis, the stimulation of the cartilage regeneration was more effective when the MSCs were administered to the MCH carrier. The present study demonstrates the way to regulate the action of the MSCs in the area of cartilage regeneration: the MCH is more conducive to stimulating cartilage repair by the MSCs, while the DPC is an inducer for a formation of a cartilage-like tissue by the MSCs in vitro.

【 授权许可】

Unknown