Fourier Transform Infrared Microspectroscopy Combined with Principal Component Analysis and Artificial Neural Networks for the Study of the Effect of β-Hydroxy-β-Methylbutyrate (HMB) Supplementation on Articular Cartilage

International Journal of Molecular Sciences

Carina Prein¹  Hauke Clausen-Schaumann¹  Tomasz Blicharski²  Katarzyna Kras³  Marcin B. Arciszewski³  Ewa Tomaszewska⁴  Michał Świetlicki⁵  Marta Arczewska⁶  Izabela Świetlicka⁶  Siemowit Muszyński⁶  Mariusz Gagoś⁷  Piotr Dobrowolski⁸  Attila Aszodi⁹ 

[1] Center for Applied Tissue Engineering and Regenerative Medicine-CANTER, Munich University of Applied Sciences, 80335 Munich, Germany;Chair and Department of Rehabilitation and Orthopedics, Medical University in Lublin, 20-090 Lublin, Poland;Department of Animal Anatomy and Histology, University of Life Sciences in Lublin, 20-950 Lublin, Poland;Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-950 Lublin, Poland;Department of Applied Physics, Faculty of Mechanical Engineering, Lublin University of Technology, 20-618 Lublin, Poland;Department of Biophysics, Faculty of Environmental Biology, University of Life Sciences in Lublin, 20-950 Lublin, Poland;Department of Cell Biology, Maria Curie Sklodowska University, 20-031 Lublin, Poland;Department of Functional Anatomy and Cytobiology, Faculty of Biology and Biotechnology, Maria Curie-Sklodowska University, 20-033 Lublin, Poland;Laboratory of Cartilage Development, Diseases and Regeneration, Department for Orthopaedics and Trauma Surgery, Musculoskeletal University Centre Munich (MUM), University Hospital, LMU Munich, 82152 Planegg, Germany;

关键词: FTIR microspectroscopy;    atomic force microscopy;    β-hydroxy-β-methylbutyrate supplementation;    animal model;    articular cartilage;    collagen;   

DOI  :  10.3390/ijms22179189

来源: DOAJ

【 摘 要 】

The potential of Fourier Transform infrared microspectroscopy (FTIR microspectroscopy) and multivariate analyses were applied for the classification of the frequency ranges responsible for the distribution changes of the main components of articular cartilage (AC) that occur during dietary β-hydroxy-β-methyl butyrate (HMB) supplementation. The FTIR imaging analysis of histological AC sections originating from 35-day old male piglets showed the change in the collagen and proteoglycan contents of the HMB-supplemented group compared to the control. The relative amount of collagen content in the superficial zone increased by more than 23% and in the middle zone by about 17%, while no changes in the deep zone were observed compared to the control group. Considering proteoglycans content, a significant increase was registered in the middle and deep zones, respectively; 62% and 52% compared to the control. AFM nanoindentation measurements collected from animals administered with HMB displayed an increase in AC tissue stiffness by detecting a higher value of Young’s modulus in all investigated AC zones. We demonstrated that principal component analysis and artificial neural networks could be trained with spectral information to distinguish AC histological sections and the group under study accurately. This work may support the use and effectiveness of FTIR imaging combined with multivariate analyses as a quantitative alternative to traditional collagenous tissue-related histology.

【 授权许可】

Unknown