The Application of Microfluidic Technologies in Aptamer Selection

Frontiers in Cell and Developmental Biology

Chiu-Wing Chan¹  Nijia Wang²  Yang Liu²  Aiping Lu⁴  Ge Zhang⁴  Yuanyuan Yu⁴  Kangning Ren⁵ 

[1] Department of Chemistry, Hong Kong Baptist University, Kowloon, Hong Kong, SAR China;Guangdong-Hong Kong Macao Greater Bay Area International Research Platform for Aptamer-Based Translational Medicine and Drug Discovery, Hong Kong, Hong Kong, SAR China;Institute of Research and Continuing Education, Hong Kong Baptist University, Shenzhen, China;School of Chinese Medicine, Law Sau Fai Institute for Advancing Translational Medicine in Bone and Joint Diseases, Hong Kong Baptist University, Hong Kong, Hong Kong, SAR China;State Key Laboratory of Environmental and Biological Analysis, Hong Kong Baptist University, Kowloon, Hong Kong, SAR China;

关键词: aptamer;    oligonucleotide;    SELEX;    microfluidics;    microarray;   

DOI  :  10.3389/fcell.2021.730035

来源: DOAJ

【 摘 要 】

Aptamers are sequences of single-strand oligonucleotides (DNA or RNA) with potential binding capability to specific target molecules, which are increasingly used as agents for analysis, diagnosis, and medical treatment. Aptamers are generated by a selection method named systematic evolution of ligands by exponential enrichment (SELEX). Numerous SELEX methods have been developed for aptamer selections. However, the conventional SELEX methods still suffer from high labor intensity, low operation efficiency, and low success rate. Thus, the applications of aptamer with desired properties are limited. With their advantages of low cost, high speed, and upgraded extent of automation, microfluidic technologies have become promising tools for rapid and high throughput aptamer selection. This paper reviews current progresses of such microfluidic systems for aptamer selection. Comparisons of selection performances with discussions on principles, structure, operations, as well as advantages and limitations of various microfluidic-based aptamer selection methods are provided.

【 授权许可】

Unknown