Proliferation of Osteoblasts on Laser-Modified Nanostructured Titanium Surfaces

Materials

Jan Cerny¹  Michal Duchek¹  Jan Palan¹  Vaclav Babuska²  Jana Kolaja Dobra²  Vlastimil Kulda²  Daniel Hrusak³ 

[1] COMTES FHT a.s., Prumyslova 995, 334 41 Dobrany, Czech Republic;Department of Medical Chemistry and Biochemistry, Faculty of Medicine in Pilsen, Charles University, Karlovarska 48, 301 66 Pilsen, Czech Republic;Department of Stomatology, Faculty of Medicine in Pilsen, University Hospital and Charles University, alej Svobody 80, 301 00 Pilsen, Czech Republic;

关键词: biocompatibility;    nanostructured titanium;    osteoblasts proliferation;    laser treatment;   

DOI  :  10.3390/ma11101827

来源: DOAJ

【 摘 要 】

Nanostructured titanium has become a useful material for biomedical applications such as dental implants. Certain surface properties (grain size, roughness, wettability) are highly expected to promote cell adhesion and osseointegration. The aim of this study was to compare the biocompatibilities of several titanium materials using human osteoblast cell line hFOB 1.19. Eight different types of specimens were examined: machined commercially pure grade 2 (cpTi2) and 4 (cpTi4) titanium, nanostructured titanium of the same grades (nTi2, nTi4), and corresponding specimens with laser-treated surfaces (cpTi2L, cpTi4L, nTi2L, nTi4L). Their surface topography was evaluated by means of scanning electron microscopy. Surface roughness was measured using a mechanical contact profilometer. Specimens with laser-treated surfaces had significantly higher surface roughness. Wettability was measured by the drop contact angle method. Nanostructured samples had significantly higher wettability. Cell proliferation after 48 hours from plating was assessed by viability and proliferation assay. The highest proliferation of osteoblasts was found in nTi4 specimens. The analysis of cell proliferation revealed a difference between machined and laser-treated specimens. The mean proliferation was lower on the laser-treated titanium materials. Although plain laser treatment increases surface roughness and wettability, it does not seem to lead to improved biocompatibility.

【 授权许可】

Unknown