Identification of Di/Tripeptide(s) With Osteoblasts Proliferation Stimulation Abilities of Yak Bone Collagen by in silico Screening and Molecular Docking

Frontiers in Nutrition

Lingyun Chen¹  Yongkai Chen¹  Feng Huang²  Chunhui Zhang²  Yujie Guo²  Yusi Liu³ 

[1] Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada;Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China;Wageningen Food and Biobased Research, Wageningen University and Research, Wageningen, Netherlands;

关键词: yak bone collagen;    di/tripeptides;    osteoblasts proliferation;    in silico screening;    molecular docking;   

DOI  :  10.3389/fnut.2022.874259

来源: DOAJ

【 摘 要 】

Endothelial protein C receptor (EPCR), cannabinoid receptor 2 (CBR2), and estrogen receptor α (ERα) play vital roles in osteoblasts proliferation. Also, collagen peptides have osteoblasts proliferation stimulation abilities, and di/tri-peptides could be absorbed by the intestine more easily. This study obtained three di/tripeptides with potential osteoblasts proliferation stimulation abilities of yak bone collagen, namely, MGF, CF, and MF, by in silico screening. Results suggested that these three peptides exhibited good absorption, distribution, metabolism, excretion, and toxicity (ADMET) properties. They also had strong affinities with EPCR, CBR2, and ERα, and the total -CDOCKER energy (-CE) values were 150.9469, 113.1835, and 115.3714 kcal/mol, respectively. However, further Cell Counting Kit-8 (CCK-8) assays indicated that only MGF could significantly (P < 0.05) stimulate osteoblasts proliferation at 0.3 mg/ml. At the same time, the proliferating index (PI) of the osteoblasts treated with MGF increased significantly (P < 0.05), and the alkaline phosphatase (ALP) activity decreased highly significantly (P < 0.01). In summary, MGF exhibited the potential to be an effective treatment for osteoporosis.

【 授权许可】

Unknown