Genome-Wide Investigation of G6PDH Gene in Strawberry: Evolution and Expression Analysis during Development and Stress

International Journal of Molecular Sciences

Diya Lei¹  Wantian Yao¹  Bing Zhao¹  Xuan Zhou¹  Ya Luo¹  Qing Chen¹  Yong Zhang¹  Yuanxiu Lin¹  Xiaorong Wang¹  Mengwen Luo¹  Yunting Zhang¹  Mengyao Li¹  Yan Wang¹  Haoru Tang¹  Honglan Tang¹ 

[1] College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China;

关键词: strawberry;    G6PDH;    genome-wide;    expression patterns;    abiotic stress;   

DOI  :  10.3390/ijms23094728

来源: DOAJ

【 摘 要 】

As one of the key enzymes in the pentose phosphate pathway (PPP), glucose-6-phosphate dehydrogenase (G6PDH) provides NADPH and plays an important role in plant development and stress responses. However, little information was available about the G6PDH genes in strawberry (Fragaria × ananassa). The recent release of the whole-genome sequence of strawberry allowed us to perform a genome-wide investigation into the organization and expression profiling of strawberry G6PDH genes. In the present study, 19 strawberry G6PDH genes (FaG6PDHs) were identified from the strawberry genome database. They were designated as FaG6PDH1 to FaG6PDH19, respectively, according to the conserved domain of each subfamily and multiple sequence alignment with Arabidopsis. According to their structural and phylogenetic features, the 19 FaG6PDHs were further classified into five types: Cy, P1, P1.1, P2 and PO. The number and location of exons and introns are similar, suggesting that genes of the same type are very similar and are alleles. A cis-element analysis inferred that FaG6PDHs possessed at least one stress-responsive cis-acting element. Expression profiles derived from transcriptome data analysis exhibited distinct expression patterns of FaG6PDHs genes in different developmental stages. Real-time quantitative PCR was used to detect the expression level of five types FaG6PDHs genes and demonstrated that the genes were expressed and responded to multiple abiotic stress and hormonal treatments.

【 授权许可】

Unknown