Moringa oleifera leaf fractions attenuated Naje haje venom-induced cellular dysfunctions via modulation of Nrf2 and inflammatory signalling pathways in rats

Biochemistry and Biophysics Reports

Babafemi Siji Ajisebiola¹  Akindele Oluwatosin Adeyi²  Peter Ofemile Yusuf³  Babajide O. Ajayi⁴  Olarewaju Adekunle⁴  Adewale Segun James⁵  Olanike Busirat Akande⁵  Babatunde A. Idowu⁶  Olubisi Esther Adeyi⁷ 

[1] Corresponding author. Tel.: [phone].;Animal Physiology Unit, Department of Zoology, University of Ibadan, Nigeria;Department of Biochemistry, Bowen University, Iwo, Nigeria;Department of Biochemistry, Federal University of Agriculture, Abeokuta, Nigeria;Department of Pure and Applied Zoology, Federal University of Agriculture, Abeokuta, Nigeria;Department of Veterinary Pharmacology and Toxicology, Ahmadu Bello University, Zaria, Nigeria;Department of Zoology, Osun State University, Osogbo, Nigeria;

关键词: Egyptian cobra;    Oxidative stress;    Nrf2;    Antivenom;    Moringa oleifera;    Inflammation;   

DOI  :  

来源: DOAJ

【 摘 要 】

Naja haje envenoming could activate multiple pathways linked to haematotoxic, neurological, and antioxidant systems dysfunctions. Moringa oleifera has been used in the management of different snake venom-induced toxicities, but there is no scientific information on its antivenom effects against Naja haje. This study thus, investigated the antivenom activities of different extract partitions of M. oleifera leaves against N. haje envenoming. Forty five male rats were divided into nine groups (n = 5). Groups 2 to 9 were envenomed with 0.025 mg/kg (LD50) of N. haje venom while group 1 was given saline. Group 2 was left untreated, while group 3 was treated with polyvalent antivenom, groups 4, 6 and 8 were treated with 300 mg/kg−1 of N-hexane, ethylacetate and ethanol partitions of M. oleifera, respectively. Groups 5, 7 and 9 were also treated with 600 mgkg−1of the partitions, respectively. Ethanol extract and ethyl acetate partition of M. oleifera significantly improved haematological indices following acute anaemia induced by the venom. Likewise, haemorrhagic, haemolytic and anti-coagulant activities of N. haje venom were best inhibited by ethanol partition. Envenoming significantly down-regulated Nuclear factor erythroid 2-related factor 2 (Nrf2) with the consequent elevation of antioxidant enzymes activities in the serum and brain. Treatment with extract partitions however, elevated Nrf2 levels while normalising antioxidant enzyme activities. Furthermore, there were reduction in levels of pro-inflammatory cytokines (TNF-α and interleukin-1β) in tissues of treated envenomed rats. This study concludes that ethanol partition of M. oleifera was most effective against N. haje venom and could be considered as a potential source for antivenom metabolites.

【 授权许可】

Unknown