Chromatographic Analyses, In Vitro Biological Activities, and Cytotoxicity of Cannabis sativa L. Essential Oil: A Multidisciplinary Study

Molecules

Valentina Puca¹  Romina Mancinelli²  Gokhan Zengin³  Marianna D’Antonio⁴  Luigi Menghini⁵  Simone Carradori⁵  Marcello Locatelli⁵  Rossella Grande⁵  Antonella Di Sotto⁶  Letizia Angiolella⁷  Caterina Fraschetti⁸  Luisa Mannina⁸  Cinzia Ingallina⁸  Antonello Filippi⁸  Stefania Cesa⁸  Francesca Sisto⁹ 

[1] CeSI-MeT Centro Scienze dell’Invecchiamento e Medicina Traslazionale, Center of Aging Sciences and Translational Medicine, 66100 Chieti, Italy;Department of Anatomical, Histological, Forensic and Orthopedic Sciences, Sapienza University of Rome, 00185 Rome, Italy;Department of Biology, Science Faculty, Selcuk University, 42130 Konya, Turkey;Department of Clinical Microbiology and Virology, Spirito Santo Hospital Pescara, 65124 Pescara, Italy;Department of Pharmacy, University “G. d’Annunzio” of Chieti-Pescara, 66100 Chieti, Italy;Department of Physiology and Pharmacology “V. Erspamer”, Sapienza University of Rome, 00185 Rome, Italy;Department of Public Health and Infectious Diseases, Sapienza University of Rome, 00161 Rome, Italy;Dipartimento di Chimica e Tecnologie del Farmaco, Sapienza Università di Roma, 00185 Rome, Italy;Dipartimento di Scienze Biomediche, Chirurgiche ed Odontoiatriche, University of Milan, 20122 Milan, Italy;

关键词: Cannabis sativa L.;    chromatographic analysis;    essential oil;    antimicrobial activity;    cancer cell cytotoxicity;    naringenin;    biofilm;    antioxidant activity;    Galleria mellonella;   

DOI  :  10.3390/molecules23123266

来源: DOAJ

【 摘 要 】

Due to renewed interest in the cultivation and production of Italian Cannabis sativa L., we proposed a multi-methodological approach to explore chemically and biologically both the essential oil and the aromatic water of this plant. We reported the chemical composition in terms of cannabinoid content, volatile component, phenolic and flavonoid pattern, and color characteristics. Then, we demonstrated the ethnopharmacological relevance of this plant cultivated in Italy as a source of antioxidant compounds toward a large panel of enzymes (pancreatic lipase, α-amylase, α-glucosidase, and cholinesterases) and selected clinically relevant, multidrug-sensible, and multidrug-resistant microbial strains (Staphylococcus aureus, Helicobacter pylori, Candida, and Malassezia spp.), evaluating the cytotoxic effects against normal and malignant cell lines. Preliminary in vivo cytotoxicity was also performed on Galleria mellonella larvae. The results corroborate the use of this natural product as a rich source of important biologically active molecules with particular emphasis on the role exerted by naringenin, one of the most important secondary metabolites.

【 授权许可】

Unknown