Chemical Composition, Cytotoxic and Antimicrobial Activity of Essential Oils from Cassia bakeriana Craib. against Aerobic and Anaerobic Oral Pathogens

Molecules

Luís C. S. Cunha¹  Sérgio A. L. de Morais¹  Carlos H. G. Martins³  Mário M. Martins¹  Roberto Chang¹  Francisco J. T. de Aquino¹  Alberto de Oliveira¹  Thaís da S. Moraes³  Fabrໜio C. Machado²  Cláudio V. da Silva² 

[1] Laboratory of Natural Products and Chromatography, Chemistry Institute, Federal University of Uberlândia, Uberlândia, 38408-144, Brazil;Institute of Biomedical Sciences, Laboratory of Molecular Biology, Federal University of Uberlândia, Uberlândia, 38400-902, Brazil;Nucleus of Research in Sciences and Technology, Laboratory of Research in Applied Microbiology (LaPeMA), University of Franca, Franca, 14404-600, Brazil

关键词: Cassia bakeriana;    essential oil;    antimicrobial activity;    cytotoxicity;   

DOI  :  10.3390/molecules18044588

来源: mdpi

【 摘 要 】

The chemical composition of the essential oils from leaves, bark and wood of Cassia bakeriana Craib. was determined by gas chromatography (GC) and gas chromatography–mass spectrometry (GC-MS). Alcohols, aldehydes and fatty acids were the major components in leaf and bark oil, while wood essential oil was rich in fatty acids. Terpenes such as linalool, (E)-nerolidol and phytol were present in low concentrations. The antimicrobial activity against aerobic and anaerobic oral bacteria was evaluated using the microdilution method, as was the cell viability test carried out with Vero cells. The oils from leaves and bark showed high antimicrobial activity, with minimum inhibitory concentrations between 62.5 and 125 µg·mL⁻¹ for most of the tested bacteria, including Streptococcusmutans, the main etiological agent of dental caries. Leaves oil displayed the lowest cytotoxic effect (EC₅₀ of 153 µg·mL⁻¹), while wood oil exhibited the highest toxicity to Vero cells. C. bakeriana oils are thus a source of biologically active compounds against aerobic and anaerobic oral microorganisms. This study is the first report on the chemical composition, antimicrobial activity and cytotoxicity of C.bakeriana.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.