DICER1-AS1 Promotes the Malignant Behaviors of Colorectal Cancer Cells by Regulating miR-296-5p/STAT3 Axis

Cancer Management and Research

关键词: dicer1-as1;    mir-296-5p;    crc;    proliferation;    metastasis;   

DOI  :  

来源: DOAJ

【 摘 要 】

Chuanyu Ma,1 Ning Ma,1 Lili Qin,1 Chuanna Miao,1 Minglei Luo,1 Shuhong Liu2 1Department of Proctology, Linyi Central Hospital, Linyi, Shandong Province, People’s Republic of China; 2Department of Radiotherapy, Linyi Cancer Hospital, Linyi, Shandong Province, People’s Republic of ChinaCorrespondence: Shuhong LiuDepartment of Radiotherapy, Linyi Cancer Hospital, Lingyuan East Street No. 6, Lanshan District, Linyi 276400, Shandong Province, People’s Republic of ChinaEmail [e-mail]: Long non-coding RNA (lncRNA) exerts a regulatory role in the occurrence and progression of tumors. This study aimed at probing into the function and mechanism of lncRNA DICER1 antisense RNA 1 (DICER1-AS1) in colorectal cancer (CRC).Methods: The expressions of DICER1-AS1, miR-296-5p and STAT3 mRNA were tested by quantitative real-time polymerase chain reaction (qRT-PCR). Cell counting kit-8 (CCK-8) assay was employed to detect cell proliferation, and Transwell was used to detect cell migration and invasion. In addition, the expressions of apoptosis-related proteins Bax and Bcl2 were detected by Western blot. Interactions between DICER1-AS1 and miR-296-5p, and miR-296-5p and STAT3 were predicted and determined by bioinformatics analysis, luciferase reporter assay and RNA binding protein immunoprecipitation (RIP) assay.Results: The expressions of DICER1-AS1 and STAT3 mRNA were significantly up-regulated while miR-296-5p expression was remarkably down-regulated in CRC tissues and cell lines. Over-expression of DICER1-AS1 or transfection of miR-296-5p inhibitors could promote the proliferation, migration and invasion and inhibit apoptosis of CRC cells, whereas knockdown of DICER1-AS1 or transfection of miR-296-5p mimics had the opposite effects. Additionally, DICER1-AS1 could down-regulate miR-296-5p expression via sponging it. DICER1-AS1 also enhanced the expression of STAT3, which was identified as a target gene of miR-296-5p.Conclusion: DICER1-AS1 acts as an oncogenic lncRNA in CRC via modulating miR-296-5p/STAT3 axis. Our results provide a new direction for the diagnosis and treatment of CRC.Keywords: DICER1-AS1, miR-296-5p, CRC, proliferation, metastasis

【 授权许可】

Unknown