Regulatory mechanism on enhancing protein synthesis in skeletal muscles of cold exposed fresh water fish (Channa punctata)

Journal of the Saudi Society of Agricultural Sciences

Md. Shahidul Haque¹  M.M.H. Khan¹  Swapan Kumar Roy¹  Md. Asraful Haque²  Md. Mosharrof Hossain³ 

[1] Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi 6205, Bangladesh;Department of Biotechnology, BSMRAU, Salna, Gazipur, Bangladesh;Department of Zoology, University of Rajshahi, Rajshahi 6205, Bangladesh;

关键词: Arsenic exposure;    Channa;    Low temperature;    Skeletal muscle;    Metabolic regulation;   

DOI  :  10.1016/j.jssas.2013.04.001

来源: DOAJ

【 摘 要 】

Channa punctata varieties of fish are energetic and survive in critical environment although the molecular mechanism is not known. They were exposed to cold (4–8 °C) for 30 min, 1 h, 2 h and 4 h and the total protein contents in the liver were not significantly changed up to 4 h of cold exposure while a significantly increased protein level in the skeletal muscle was noted and maximal at 2 h. Groups of fish were exposed to Na2HAsO4 to examine its role on cold-induced protein synthesis in the skeletal muscle and the increased protein in the skeletal muscle was reduced significantly. The results appear to indicate that cold acclimation induces a metabolic change involving cellular protein content tissue specifically and arsenic might be involved in impairment of the cold-induced effect. To clarify the molecular mechanism, groups of fish exposed to cold for 1 h and 2 h had significantly increased RNA in the skeletal muscle compared to control fish, however, a higher level was found after 2 h of treatment and the enhanced RNA induced by cold was almost completely prevented by Na2HAsO4. Our findings will give a new insight into the survival process of this species while toxic arsenic prevents this cellular bioprocess.

【 授权许可】

Unknown