Optimized Extraction of Polysaccharides from Grateloupia livida (Harv.) Yamada and Biological Activities

Molecules

Ganggang Shi¹  Hongmei Wang¹  Danyan Ye¹  Yanmei Zhang¹  Peihong Chen¹  Fenfei Gao¹  Yicun Chen¹  Zebin Jiang²  Fuchun Zheng² 

[1] Department of Pharmacology, Shantou University Medical College, Shantou 515041, China;Department of Pharmacy, First Affiliated Hospital, Shantou University Medical College,Shantou 515041, China;

关键词: Grateloupia livida (Harv.) Yamada;    polysaccharide extraction;    Box-Behnken design;    antioxidant and anti-cancer activity;   

DOI  :  10.3390/molecules200916817

来源: DOAJ

【 摘 要 】

Polysaccharides from Grateloupia livida (Harv.) Yamada (GL) were extracted by a heating circumfluence method. Single-factor experiments were performed for the three parameters: extraction time (X1), extraction temperature (X2) and the ratio of water to raw material (X3) and their test range. From preliminary experimental results, one type of the response surface methodology, the Box-Behnken design was applied for the optimizing polysaccharide extraction conditions. The experimental data obtained were fitted to a second-order polynomial equation. The optimal conditions were extraction time 5 h, extraction temperature 100 °C and ratio of water to raw material 70 mL/g. Under these conditions, the experimental yield was 39.22% ± 0.09%, which well matched the predicted value (39.25%), with 0.9774 coefficient of determination (R2). GL polysaccharides had scavenging activities for DPPH and hydroxyl radicals in vitro. The scavenging rates for both radicals peaked at 20 mg/mL GL concentration. However, the positive standard, VC (ascorbic acid), possessed stronger antioxidant activities than GL polysaccharides. Furthermore, the anticancer activity of GL polysaccharides on HepG2 cell proliferation increased dose- and time-dependently, but the positive standard, 5-fluorouracil (5-fu) showed more significant anticancer activity in this study. Overall, GL polysaccharides may have potential applications in the medical and food industries.

【 授权许可】

Unknown