Optimized Extraction of Polysaccharides from Grateloupia livida (Harv.) Yamada and Biological Activities

Molecules

Danyan Ye¹  Zebin Jiang²  Fuchun Zheng²  Hongmei Wang¹  Yanmei Zhang¹  Fenfei Gao¹  Peihong Chen¹  Yicun Chen¹  Ganggang Shi¹ 

[1] Department of Pharmacology, Shantou University Medical College, Shantou 515041, China; E-Mails:;Department of Pharmacy, First Affiliated Hospital, Shantou University Medical College, Shantou 515041, China; E-Mails:

关键词: Grateloupia livida (Harv.) Yamada;    polysaccharide extraction;    Box-Behnken design;    antioxidant and anti-cancer activity;   

DOI  :  10.3390/molecules200916817

来源: mdpi

【 摘 要 】

Polysaccharides from Grateloupia livida (Harv.) Yamada (GL) were extracted by a heating circumfluence method. Single-factor experiments were performed for the three parameters: extraction time (X₁), extraction temperature (X₂) and the ratio of water to raw material (X₃) and their test range. From preliminary experimental results, one type of the response surface methodology, the Box-Behnken design was applied for the optimizing polysaccharide extraction conditions. The experimental data obtained were fitted to a second-order polynomial equation. The optimal conditions were extraction time 5 h, extraction temperature 100 °C and ratio of water to raw material 70 mL/g. Under these conditions, the experimental yield was 39.22% ± 0.09%, which well matched the predicted value (39.25%), with 0.9774 coefficient of determination (R²). GL polysaccharides had scavenging activities for DPPH and hydroxyl radicals in vitro. The scavenging rates for both radicals peaked at 20 mg/mL GL concentration. However, the positive standard, VC (ascorbic acid), possessed stronger antioxidant activities than GL polysaccharides. Furthermore, the anticancer activity of GL polysaccharides on HepG2 cell proliferation increased dose- and time-dependently, but the positive standard, 5-fluorouracil (5-fu, showed more significant anticancer activity in this study. Overall, GL polysaccharides may have potential applications in the medical and food industries.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.