Loop Mediated Isothermal Amplification: Principles and Applications in Plant Virology

Plants

Antonio Tiberini¹  Raffaele Stassi²  Salvatore Davino²  Stefano Panno²  AndreaGiovanni Caruso²  Patrizia Bella²  Livio Torta²  Slavica Matić³ 

[1] Council for Agricultural Research and Economics, Research Center for Plant Protection and Certification, 00156 Rome, Italy;Department of Agricultural, Food and Forest Sciences, University of Palermo, 90128 Palermo, Italy;Department of Agricultural, Forestry and Food Sciences, University of Turin, 10095 Turin, Italy;

关键词: loop-mediated isothermal amplification;    LAMP;    Bst DNA polymerase;    primers;    real-time monitoring;    virus;   

DOI  :  10.3390/plants9040461

来源: DOAJ

【 摘 要 】

In the last decades, the evolution of molecular diagnosis methods has generated different advanced tools, like loop-mediated isothermal amplification (LAMP). Currently, it is a well-established technique, applied in different fields, such as the medicine, agriculture, and food industries, owing to its simplicity, specificity, rapidity, and low-cost efforts. LAMP is a nucleic acid amplification under isothermal conditions, which is highly compatible with point-of-care (POC) analysis and has the potential to improve the diagnosis in plant protection. The great advantages of LAMP have led to several upgrades in order to implement the technique. In this review, the authors provide an overview reporting in detail the different LAMP steps, focusing on designing and main characteristics of the primer set, different methods of result visualization, evolution and different application fields, reporting in detail LAMP application in plant virology, and the main advantages of the use of this technique.

【 授权许可】

Unknown