Taxonomic and Bioactivity Characterizations of Mameliella alba Strain LZ-28 Isolated from Highly Toxic Marine Dinoflagellate Alexandrium catenella LZT09

Marine Drugs

Hui-Min Gao¹  Cheng-Zhe Ren¹  Xiao-Ling Zhang¹  Wen-Zhuo Zhu¹  Qiao Yang¹  Jun Dai²  Fei-Fei Xu³  Yun Ye⁴ 

[1] Department of Marine Chemistry, College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China;Natural “111” Center for Cellular Regulation and Molecular Pharmaceutics, Key Laboratory of Fermentation Engineering, Ministry of Education, College of Bioengineering, Hubei University of Technology, Wuhan 430068, China;Zhejiang Yimeiyuan Testing Technology Co., Ltd., Zhoushan 316022, China;Zhoushan Natural Resources Surveying and Mapping Design Centre, Zhoushan 316021, China;

关键词: bacterial exopolysaccharides;    bioflocculanting activity;    microalgae growth-promoting bacterium;    harmful algal bloom-forming dinoflagellate;    Alexandrium catenella;    Mameliella alba;   

DOI  :  10.3390/md20050321

来源: DOAJ

【 摘 要 】

Microalgae host varied microbial consortium harboring cross-kingdom interactions with fundamental ecological significance in aquatic ecosystems. Revealing the complex biofunctions of the cultivable bacteria of phycosphere microbiota is one vital basis for deeply understanding the mechanisms governing these dynamic associations. In this study, a new light-yellow pigmented bacterial strain LZ-28 was isolated from the highly-toxic and harmful algal bloom-forming dinoflagellate Alexandrium catenella LZT09. Collective phenotypic and genotypic profiles were obtained to confidently identify this strain as a new Mameliellaalba member. Comparative genomic analysis showed that strain LZ-28 shared highly similar functional features with other four marine algae-derived M. alba strains in spite of their distinctive isolation sources. Based on the bioactivity assaying, the mutual growth-promoting effects between bacterial strain LZ-28 and algal strain LZT09 were observed. After the culture conditions were optimized, strain LZ-28 demonstrated an extraordinary production ability for its bioflocculanting exopolysaccharides (EPS). Moreover, the portions of two monosaccharides glucose and fucose of the EPS were found to positively contribute to the bioflocculanting capacity. Therefore, the present study sheds light on the similar genomic features among the selected M. alba strains, and it also reveals the potential pharmaceutical, environmental and biotechnological implications of active EPS produced by this new Mameliella alba strain LZ-28 recovered from toxic bloom-forming marine dinoflagellate.

【 授权许可】

Unknown