Evaluation of an O2-Substituted (1–3)-β-D-Glucan, Produced by Pediococcus parvulus 2.6, in ex vivo Models of Crohn’s Disease

Frontiers in Microbiology

Encarnación Varela¹  Anna Otal²  María Antolín²  Francisco Guarner²  Alicia Prieto³  Iván Cristobo³  Paloma López³  Sara Notararigo⁴ 

[1] CIBERehd, Instituto de Salud Carlos III, Madrid, Spain;Department of Gastroenterology, Digestive System Research Unit, Institut de Recerca Vall d’Hebron (VHIR), University Hospital Vall d’Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain;Department of Microbial: and Plant Biotechnology, Margarita Salas Biological Research Centre (CIB-Margarita Salas-CSIC), Madrid, Spain;Foundation Health Research Institute of Santiago de Compostela (FIDIS), Santiago de Compostela, Spain;

关键词: bacterial exopolysaccharides;    O2-substituted-(1-3)-β-D-glucan;    lactic acid bacteria;    Immunomodulation;    Crohn´s disease anti-inflammatory effect;   

DOI  :  10.3389/fmicb.2021.621280

来源: DOAJ

【 摘 要 】

1,3-β-glucans are extracellular polysaccharides synthesized by microorganisms and plants, with therapeutic potential. Among them, the O2-substituted-(1–3)-β-D-glucan, synthesized by some lactic acid bacteria (LAB), has a prebiotic effect on probiotic strains, an immunomodulatory effect on monocyte-derived macrophages, and potentiates the ability of the producer strain to adhere to Caco-2 cells differentiated to enterocytes. In this work, the O2-substituted-(1–3)-β-D-glucan polymers produced by GTF glycoyltransferase in the natural host Pediococcus parvulus 2.6 and in the recombinant strain Lactococcus lactis NZ9000[pNGTF] were tested. Their immunomodulatory activity was investigated in an ex vivo model using human biopsies from patients affected by Crohn’s disease (CD). Both polymers had an anti-inflammatory effect including, a reduction of Interleukine 8 both at the level of its gene expression and its secreted levels. The overall data indicate that the O2-substituted-(1–3)-β-D-glucan have a potential role in ameliorating inflammation via the gut immune system cell modulation.

【 授权许可】

Unknown