Optimizing imaging depth of anisotropic scattering tissues with polarization engineered second harmonic generation microscopy

Results in Physics

Ming-Xin Lee¹  Zhi-Teng Su²  Guan-Yu Zhuo³  Fu-Jen Kao³  Shuai-Yan Chen⁴  Ming-Che Chan⁵  Subir Das⁶  Dan-Jae Lin⁷ 

[1] The Ph.D. Program for Medical Engineering and Rehabilitation Science, China Medical University, Taichung 40402, Taiwan;Graduate Institute of Biomedical Sciences, China Medical University, Taichung 40402, Taiwan;Institute of Biophotonics, National Yang Ming Chiao Tung University, Taipei 11221, Taiwan;Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 80424, Taiwan;Institute of New Drug Development, China Medical University, Taichung 40402, Taiwan;Institute of Photonic System, College of Photonics, National Yang Ming Chiao Tung University, Tainan 71150, Taiwan;School of Dentistry, College of Dentistry, China Medical University, Taichung 40402, Taiwan;

关键词: Second harmonic generation;    Polarization;    Signal-to-background ratio;    Imaging depth;    Multiple scattering;    Birefringence;   

DOI  :  

来源: DOAJ

【 摘 要 】

Connective tissues in vertebrates consist of many anisotropic structures formed by collagen and muscle fibers, which could also generate intense second harmonic (SH). In SHG based tissue imaging, the incident light, when subjected to birefringence and scattering, would lead to a rapid decrement in imaging depth. The work simulating polarized light propagating through a thick and highly-scattering semi-infinite medium using a polarization-sensitive Monte Carlo model find that circular polarization would achieve deeper penetration depth. Henceforth, we use polarization engineered SHG imaging to investigate fish scales and pig tendon/dermis of various thickness, as well as the corresponding depolarization effect as a function of the imaging depth in this work. Critically, we have verified quantitatively the previous simulation results and presented the possibility to greatly improve the imaging of thick anisotropic and scattering tissues through engineering polarization. In parallel to wavefront shaping that uses a spatial light modulator or a wavefront sensor based deformable mirror to increase the signal-to-background (SBR) ratio in imaging, our approach is simple, effective, and sensitive to tissue anisotropy.

【 授权许可】

Unknown