Different PPD-stimulated cytokine responses from patients infected with genetically distinct Mycobacterium tuberculosis complex lineages

International Journal of Infectious Diseases

Voahangy Rasolofo¹  Niaina Rakotosamimanana²  Marie Sylvianne Rabodoarivelo³  Mame Diarra Bousso Ndiaye³  Paulo Ranaivomanana³ 

[1] Mycobacteria Unit, Institut Pasteur de Madagascar, B.P. Ambatofotsikely, Antananarivo, Madagascar;Corresponding author at: Mycobacteria Unit, Institut Pasteur de Madagascar, B.P. 1274, Ambatofotsikely, 101 Antananarivo, Madagascar.;Mycobacteria Unit, Institut Pasteur de Madagascar, B.P. Ambatofotsikely, Antananarivo, Madagascar;

关键词: Tuberculosis;    Mycobacterium tuberculosis complex lineages;    Cytokine;    Antigen stimulation;    Whole blood;   

DOI  :  

来源: DOAJ

【 摘 要 】

Objectives: The genetic diversity of Mycobacterium tuberculosis complex (MTBC) influences the immune response of the host, which may affect the immunodiagnostic tests and biomarker assessment studies used for tuberculosis (TB). This study aimed to determine whether the mycobacterial-antigen-stimulated cytokine responses vary with the genotype of the MTBC infecting the patient. Methods: Eighty-one patients with confirmed active pulmonary TB were recruited, and MTBC clinical strains were isolated from their sputum for bacterial lineage single-nucleotide polymorphism typing. Whole blood was drawn from the patients to measure the purified protein derivative (PPD)-stimulated cytokine responses (GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, TNF-α, IFN-α, IL-12, eotaxin, IL-13, IL-15, IL-17, MIP1-α, MIP1-β, MCP1, IL1RA, IP10, IL2R, MIG) with the Luminex multiplex immunoassay. Results: Of the 24 cytokines studied, three were produced differentially in whole blood dependent on the infecting lineage of MTBC. Decreased production of IL-17 was observed in patients infected with modern lineages compared with patients infected with ancestral lineages (P < 0.01), and production of IFN-γ and IL-2 was significantly decreased in patients infected with lineage 4 strains compared with patients infected with lineage 3 strains (P < 0.05). Conclusion: MTBC strains belonging to lineage 4 induced a decreased whole-blood PPD-stimulated pro-inflammatory cytokine response.

【 授权许可】

Unknown