Antibiofilm Activity of Phorbaketals from the Marine Sponge Phorbas sp. against Staphylococcus aureus

Marine Drugs

Young-Kyung Lee¹  Sangbum Lee¹  BuyngSu Hwang¹  Jintae Lee²  Yong-Guy Kim²  Jin-Hyung Lee² 

[1] Department of Oceanography, Kunsan National University, Kunsan 54150, Korea;School of Chemical Engineering, Yeungnam University, Gyeongsan-si 38541, Gyeongsangbukdo, Korea;

关键词: antibiofilm activity;    marine sponge;    phorbaketals;    Staphylococcus aureus;    staphyloxanthin;   

DOI  :  10.3390/md19060301

来源: DOAJ

【 摘 要 】

Biofilm formation by Staphylococcus aureus plays a critical role in the persistence of chronic infections due to its tolerance against antimicrobial agents. Here, we investigated the antibiofilm efficacy of six phorbaketals: phorbaketal A (1), phorbaketal A acetate (2), phorbaketal B (3), phorbaketal B acetate (4), phorbaketal C (5), and phorbaketal C acetate (6), isolated from the Korean marine sponge Phorbas sp. Of these six compounds, 3 and 5 were found to be effective inhibitors of biofilm formation by two S. aureus strains, which included a methicillin-resistant S. aureus. In addition, 3 also inhibited the production of staphyloxanthin, which protects microbes from reactive oxygen species generated by neutrophils and macrophages. Transcriptional analyses showed that 3 and 5 inhibited the expression of the biofilm-related hemolysin gene hla and the nuclease gene nuc1.

【 授权许可】

Unknown