Regulation of Postharvest Tomato Fruit Ripening by Endogenous Salicylic Acid

Frontiers in Plant Science

M. Z. Abdin¹  Chunoti Changwal¹  Neera Singh²  Tushita Shukla³  Virendra P. Singh³  Ajay Arora³  Abhijit Kar⁴  Zakir Hussain⁵ 

[1] Department of Biotechnology, Faculty of Science, Center for Transgenic Plant Development, Jamia Hamdard, New Delhi, India;Division of Agricultural Chemicals, ICAR - Indian Agricultural Research Institute, New Delhi, India;Division of Plant Physiology, ICAR - Indian Agricultural Research Institute, New Delhi, India;Division of Post-harvest Technology, ICAR - Indian Agricultural Research Institute, New Delhi, India;Division of Vegetable Sciences, ICAR - Indian Agricultural Research Institute, New Delhi, India;

关键词: ethylene;    postharvest;    salicylic acid;    ripening;    shelf life;    tomato;   

DOI  :  10.3389/fpls.2021.663943

来源: DOAJ

【 摘 要 】

Exogenous application of salicylic acid (SA) has been known for delaying ripening in many fruit and vegetables. But the function of endogenous SA in relation to postharvest fruit performance is still unexplored. To understand the role of endogenous SA in postharvest fruit ripening of tomato, 33 tomato lines were examined for their endogenous SA content, membrane stability index (MSI), and shelf life (SL) at turning and red stages of tomato fruit ripening. Six tomato lines having contrasting shelf lives from these categories were subjected further for ethylene (ET) evolution, 1-aminocyclopropane-1-carboxylic acid synthase (ACS), 1-aminocyclopropane-1-carboxylic acid oxidase (ACO), polygalacturonase (PG), pectin methyl esterase (PME), antioxidant assays and lipid peroxidation. It was found that high endogenous SA has a direct association with low ET evolution, which leads to the high SL of fruit. High lycopene content was also found to be correlated with high SA. Total antioxidants, PG, and PME decreased and lipid peroxidation increased from turning to red stage of tomato fruit development. Furthermore, these lines were subjected to expression analysis for SA biosynthesis enzymes viz. Solanum lycopersicum Isochorismate Synthase (SlICS) and SlPAL. Real-time PCR data revealed that high SL lines have high SlPAL4 expression and low SL lines have high SlPAL6 expression. Based on the results obtained in this study, it was concluded that endogenous SA regulates ET evolution and SL with the aid of the antioxidative defense system, and SlPAL4 and SlPAL6 genes play significant but opposite roles during fruit ripening.

【 授权许可】

Unknown