Comparative Testing of Two Ligature-Induced Periodontitis Models in Rats: A Clinical, Histological and Biochemical Study

Biology

Bogdan Crișan¹  Cristian M. Dinu²  Ștefan A. Petruțiu³  Darius C. Tomina³  Ioana A. Rațiu⁴  Vasile S. Cighi⁵ 

[1] Department of Maxillofacial Surgery and Implantology, Faculty of Dentistry, University of Medicine and Pharmacy Iuliu Hațieganu, 400012 Cluj-Napoca, Romania;Department of Oral and Cranio-Maxillofacial Surgery, Faculty of Dentistry, University of Medicine and Pharmacy Iuliu Hațieganu, 400012 Cluj-Napoca, Romania;Department of Periodontology, Faculty of Dentistry, University of Medicine and Pharmacy Iuliu Hațieganu, 400012 Cluj-Napoca, Romania;Discipline of Nephrology, Department of Medical Sciences, Faculty of Medicine and Pharmacy, University of Oradea, 410073 Oradea, Romania;Faculty of Animal Science and Biotechnologies, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, Romania;

关键词: experimental periodontitis;    systemic inflammation;    Interleukin-1alpha;    tumor necrosis factor-alpha;    high sensitive C reactive protein histology;   

DOI  :  10.3390/biology11050634

来源: DOAJ

【 摘 要 】

Experimental animal models for studying the mechanisms of periodontitis and its links are a better alternative to in vitro studies. The aim of this study is to compare two ligature induced periodontitis models and validate the best one for further use in research. An experimental study was performed on male Wistar rats that were divided into three groups: Test 1 (n = 10), incisor ligated, Test 2 (n = 10), molar ligated, and Control (n = 10). The animals were clinically evaluated at the beginning and at the end of the experiment by recording body weight, gingival bleeding index, tooth mobility score, changes in color, and consistency of gingival tissue. Two blood samples were obtained for each animal at baseline and at the end of the experiment. The hematological parameters Interleukin-1 alpha (IL-1 α), high sensitive C Reactive Protein (hsCRP), and Tumor Necrosis Factor-alpha (TNF-α) were measured. Seven days after the induction of periodontitis, the animals were sacrificed, and samples were prepared for histological evaluation. The results of this research demonstrated that the association between clinical, histological, and biochemical parameters initiate a periodontal pathology in the molar induced model in rats while the incisor experimental model initiates only a moderate and incomplete periodontal inflammation, mainly due to mechanical irritation.

【 授权许可】

Unknown