Mapping brain-wide excitatory projectome of primate prefrontal cortex at submicron resolution and comparison with diffusion tractography

eLife

Tingting Bo¹  Xiaoyu Chen²  Yilin Liu³  Zilong Qiu⁴  Wenwen Yu⁴  Qiming Hu⁴  Xiangyu Shen⁴  Xiao-Yong Zhang⁴  Fuqiang Xu⁴  Zheng Wang⁵  He Wang⁵  Mingchao Yan⁵  Yuanjing Feng⁵  Jiangli Yu⁵  Yafeng Zhan⁵  Shengyao Yan⁵  Baofeng Yang⁵  Qian Lv⁶  Qiming Lv⁷ 

[1] Beijing Key Laboratory of Behavior and Mental Health;IDG/McGovern Institute for Brain Research;Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China;University of Chinese Academy of Sciences, Beijing, China;Institute of Neuroscience, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China;Institute of Science and Technology for Brain-inspired Intelligence, Fudan University, Shanghai, China;School of Psychological and Cognitive Sciences;

关键词: macaque;    prefrontal cortex;    viral tracing;    diffusion tractography;    inferior fronto-occipital fasciculus;   

DOI  :  10.7554/eLife.72534

来源: DOAJ

【 摘 要 】

Resolving trajectories of axonal pathways in the primate prefrontal cortex remains crucial to gain insights into higher-order processes of cognition and emotion, which requires a comprehensive map of axonal projections linking demarcated subdivisions of prefrontal cortex and the rest of brain. Here, we report a mesoscale excitatory projectome issued from the ventrolateral prefrontal cortex (vlPFC) to the entire macaque brain by using viral-based genetic axonal tracing in tandem with high-throughput serial two-photon tomography, which demonstrated prominent monosynaptic projections to other prefrontal areas, temporal, limbic, and subcortical areas, relatively weak projections to parietal and insular regions but no projections directly to the occipital lobe. In a common 3D space, we quantitatively validated an atlas of diffusion tractography-derived vlPFC connections with correlative green fluorescent protein-labeled axonal tracing, and observed generally good agreement except a major difference in the posterior projections of inferior fronto-occipital fasciculus. These findings raise an intriguing question as to how neural information passes along long-range association fiber bundles in macaque brains, and call for the caution of using diffusion tractography to map the wiring diagram of brain circuits.

【 授权许可】

Unknown