Effect of Excess Gravitational Force on Cultured Myotubes in Vitro

Journal of Systemics, Cybernetics and Informatics

Haruka Hino¹  Shigehiro Hashimoto¹  Takeshi Iwagawa² 

[1] Kogakuin University;Osaka Institute of Technology;

关键词: Biomedical Engineering;    Myotube;    Gravitational Force;    Cell Culture;    fusion;    Differentiation;   

DOI  :  

来源: DOAJ

【 摘 要 】

An effect of an excess gravitational force on cultured myoblasts has been studied in an experimental system with centrifugal force in vitro. Mouse myoblasts (C2C12) were seeded on a culture dish of 35 mm diameter, and cultured in the Dulbecco's Modified Eagle's Medium until the sub-confluent condition. To apply the excess gravitational force on the cultured cells, the dish was set in a conventional centrifugal machine. Constant gravitational force was applied to the cultured cells for three hours. Variations were made on the gravitational force (6 G, 10 G, 100 G, 500 G, and 800 G) with control of the rotational speed of the rotator in the centrifugal machine. Morphology of the cells was observed with a phasecontrast microscope for eight days. The experimental results show that the myotube thickens day by day after the exposure to the excess gravitational force field. The results also show that the higher excess gravitational force thickens myotubes. The microscopic study shows that myotubes thicken with fusion each other.

【 授权许可】

Unknown