Rutin Inhibits Streptococcus suis Biofilm Formation by Affecting CPS Biosynthesis

Frontiers in Pharmacology

Ishfaq Muhammad¹  Hua Cai²  Xinmiao He²  Di Liu³  Shuai Wang⁴  Changgeng Xu⁴  Wenya Ding⁴  Jianqing Chen⁴  Xueying Chen⁴  Yanhua Li⁴  Yonghui Zhou⁴  Chang Wang⁴  Lingfei Gao⁴  Yanbei Yang⁴ 

[1] College of Veterinary Medicine, Northeast Agricultural UniversityHarbin, China;Harbin Pharmaceutical Group Bio-Vaccine Co. Ltd. (Hayao Vaccine),Harbin, China;Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Northeast Agricultural UniversityHarbin, China;Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical DevelopmentHarbin, China;

关键词: Streptococcus suis;    biofilms;    rutin;    adhesion;    CPS;   

DOI  :  10.3389/fphar.2017.00379

来源: DOAJ

【 摘 要 】

Streptococcus suis (S. suis) form biofilms and causes severe diseases in humans and pigs. Biofilms are communities of microbes embedded in a matrix of extracellular polymeric substances. Eradicating biofilms with the use of antibiotics or biocides is often ineffective and needs replacement with other potential agents. Compared to conventional agents, promising and potential alternatives are biofilm-inhibiting compounds without impairing growth. Here, we screened a S. suis adhesion inhibitor, rutin, derived from Syringa. Rutin, a kind of flavonoids, shows efficient biofilm inhibition of S. suis without impairing its growth. Capsular polysaccharides(CPS) are reported to be involved in its adherence to influence bacterial biofilm formation. We investigated the effect of rutin on S. suis CPS content and structure. The results showed that rutin was beneficial to improve the CPS content of S. suis without changing its structure. We further provided evidence that rutin specifically affected S. suis biofilm susceptibility by affecting CPS biosynthesis in vitro. The study explores the antibiofilm potential of rutin against S. suis which can be used as an adhesion inhibitor for the prevention of S. suis biofilm-related infections. Nevertheless, rutin could be used as a novel natural inhibitor of biolfilm and its molecular mechanism provide basis for its pharmacological and clinical applications.

【 授权许可】

Unknown