Viral Traits and Cellular Knock-Out Genotype Affect Dependence of BVDV on Bovine CD46

Pathogens

Kati Szakmary-Braendle¹  Verena Huber¹  Marlene Moetz¹  Hann-Wei Chen¹  Christiane Riedel¹  Till Ruemenapf¹  Kerstin Seitz¹ 

[1] Institute of Virology, Department of Pathobiology, University of Veterinary Medicine, 1210 Vienna, Austria;

关键词: bovine viral diarrhea virus;    pestivirus;    bovine CD46;    susceptibility;    CRISPR-CAS9 mediated knock-out;   

DOI  :  10.3390/pathogens10121620

来源: DOAJ

【 摘 要 】

The role of bovine CD46 in the host cell entry of BVDV has been established for more than a decade. By generating novel MDBK CD46 knock-out clones, we confirm previously reported data on the CD46 motives important for BVDV binding and the importance of the G479R exchange within BVDV E^rns to gain independence of bovine CD46 during entry. The comparison of different knock-out genotypes revealed a high variability of cellular susceptibility for a BVDV encoding the G479R exchange. These data highlight the effect of clonal selection of knock-outs on virus susceptibility, which should be considered when planning knock-out experiments.

【 授权许可】

Unknown