Identificação de microcistina LR ao nível molecular empregando microscopia de força atômica

Química Nova

Carolina de Castro Bueno¹  Augusto Etchegaray¹  Omar Teschke² 

[1] Pontifícia Universidade Católica de Campinas;Universidade Estadual de Campinas;

关键词: microcystin LR;    atomic force microscope;    biosensor development;   

DOI  :  10.1590/S0100-40422010000900004

来源: DOAJ

【 摘 要 】

Microcystins are non-ribosomal peptides that must be detected for its health concern. Here, microcystin LR and its specific antibody were respectively tethered to the substrate and to the tip of an atomic force microscope, after surface functionalization using 3-aminopropyltriethoxysilane and glutaraldehyde. Functionalization was confirmed comparing topographic images taken on bare and modified tips. Force versus distance curves were successfully used to measure the specific antibody-antigen interactions comparing with a control in which microcystin was initially blocked by incubation with free antibodies. The results showed unequivocally the specific recognition of MLR, suggesting that this method could be useful for biosensor development.

【 授权许可】

Unknown