Identification of microcistin LR at the molecular level using atomic force microscopy

Química Nova

Teschke, Omar¹  Bueno, Carolina de Castro¹  Etchegaray, Augusto¹  Pontifícia Universidade Católica de Campinas, Campinas, Brasil¹  Universidade Estadual de Campina, Campinas, Brasil¹ 

关键词: microcystin LR;    atomic force microscope;    biosensor development.;   

DOI  :  10.1590/S0100-40422010000900004

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Microcystins are non-ribosomal peptides that must be detected for its health concern. Here, microcystin LR and its specific antibody were respectively tethered to the substrate and to the tip of an atomic force microscope, after surface functionalization using 3-aminopropyltriethoxysilane and glutaraldehyde. Functionalization was confirmed comparing topographic images taken on bare and modified tips. Force versus distance curves were successfully used to measure the specific antibody-antigen interactions comparing with a control in which microcystin was initially blocked by incubation with free antibodies. The results showed unequivocally the specific recognition of MLR, suggesting that this method could be useful for biosensor development.

【 授权许可】

Unknown