Identification of microcistin LR at the molecular level using atomic force microscopy

Quimica nova

Etchegaray, Augusto¹  Bueno, Carolina de Castro¹  Teschke, Omar² 

[1] Pontifícia Universidade Católica de Campinas, Campinas, Brasil;Universidade Estadual de Campina, Campinas, Brasil

关键词: microcystin LR;    atomic force microscope;    biosensor development.;   

DOI  :  10.1590/S0100-40422010000900004

学科分类：化学（综合）

来源: Sociedade Brasileira de Quimica

【 摘 要 】

Microcystins arenon-ribosomal peptides that must be detected for its health concern. Here, microcystinLR and its specific antibody were respectively tethered to the substrate andto the tip of an atomic force microscope, after surface functionalization using3-aminopropyltriethoxysilane and glutaraldehyde. Functionalization was confirmedcomparing topographic images taken on bare and modified tips. Force versus distancecurves were successfully used to measure the specific antibody-antigen interactionscomparing with a control in which microcystin was initially blocked by incubationwith free antibodies. The results showed unequivocally the specific recognitionof MLR, suggesting that this method could be useful for biosensor development.

【 授权许可】

CC BY