A multiplexed gas exchange system for increased throughput of photosynthetic capacity measurements

Plant Methods

Thomas N. Buckley¹  Matthew E. Gilbert¹  William T. Salter² 

[1] Department of Plant Sciences, University of California, Davis;School of Life and Environmental Sciences, Sydney Institute of Agriculture, The University of Sydney;

关键词: Phenotyping;    Photosynthesis;    High-throughput;    Gas-exchange;    Photosynthetic capacity;    A max;   

DOI  :  10.1186/s13007-018-0347-y

来源: DOAJ

【 摘 要 】

Abstract Background Existing methods for directly measuring photosynthetic capacity (A max) have low throughput, which creates a key bottleneck for pre-breeding and ecological research. Currently available commercial leaf gas exchange systems are not designed to maximize throughput, on either a cost or time basis. Results We present a novel multiplexed semi-portable gas exchange system, OCTOflux, that can measure A max with approximately 4–7 times the throughput of commercial devices, despite a lower capital cost. The main time efficiency arises from having eight leaves simultaneously acclimate to saturating CO2 and high light levels; the long acclimation periods for each leaf (13.8 min on average in this study) thus overlap to a large degree, rather than occurring sequentially. The cost efficiency arises partly from custom-building the system and thus avoiding commercial costs like distribution, marketing and profit, and partly from optimizing the system’s design for A max throughput rather than flexibility for other types of measurements. Conclusion Throughput for A max measurements can be increased greatly, on both a cost and time basis, by multiplexing gas streams from several leaf chambers connected to a single gas analyzer. This can help overcome the bottleneck in breeding and ecological research posed by limited phenotyping throughput for A max.

【 授权许可】

Unknown