Evaluation of phenotyping methods for maize

Maize;Corn Root;Phenotyping

Reis, Robert ; Grift ; Tony E.

关键词: Maize;    Corn Root;    Phenotyping;   

Others  :  https://www.ideals.illinois.edu/bitstream/handle/2142/46652/Robert_Reis.pdf?sequence=1&isAllowed=y

美国|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

The quantitative measurement of plant features, also known as “phenotyping”, is animportant step in the development of improved varieties of corn. These improvements typicalconsist of increased yield, but also increased resistance to biotic and abiotic stresses is paramount.The speed of this crop improvement is currently limited by rather archaic methods ofphenotyping, often consisting of manual measurements of morphological features. In thisresearch, a new addition to the technology of phenotyping was developed. Earlier, image basedhigh-throughput phenotyping methods have been developed that allow for the measurement ofroot complexity, stalk diameter and root angle. The method used is direct imaging of the rootstructures under controlled diffuse lighting. The method as developed in this work, differs in thatthe lighting is not diffuse, but structured in the form of a laser sheet that is projected through aroot system, where the illuminated areas are recorded using a digital camera. By moving thelaser sheet to vertically different areas of the root structure were illuminated. The root was alsorotated, allowing for several sides of the root to be scanned and the resulting angle to be based onmultiple views of the root. The same procedure was applied to tassels. The imagery wasanalyzed to obtain root angles that were compared to root angles measured using the directimaging method.Important issues for future development were identified, including sample preparationprocedures, the requirements of all systems, and improvements to the technology.