Differences in the Quantity and Composition of Extracellular Vesicles in the Aqueous Humor of Patients with Retinal Neovascular Diseases

Diagnostics

Chee-Ming Lee¹  Connie Chen¹  Pei-Ying Chen¹  Min-Yen Hsu¹  Wei-Heng Chung¹  Yai-Ping Hsiao¹  Bei-Han Ko¹  Yu-Chien Hung²  Yu-Ping Wang³  Chao-Min Cheng⁴  Chihchen Chen⁵ 

[1] Department of Ophthalmology, Chung Shan Medical University Hospital, Taichung 402306, Taiwan;Department of Ophthalmology, Taichung Veterans General Hospital, Taichung 407219, Taiwan;Department of Radiology, Taichung Veterans General Hospital, Taichung 407219, Taiwan;Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu 300044, Taiwan;Institute of Nanoengineering and Microsystem, National Tsing Hua University, Hsinchu 30044, Taiwan;

关键词: extracellular vesicle;    exosome;    aqueous humor;    nanoparticle tracking analysis;    retinal neovascular disease;    angiogenesis;   

DOI  :  10.3390/diagnostics11071276

来源: DOAJ

【 摘 要 】

Extracellular vesicles (EVs) are secreted by various cells in the body fluid system and have been found to influence vessel formation and inflammatory responses in a variety of diseases. However, which EVs and their subtypes are involved in vascular retinal diseases is still unclear. Therefore, the aim of this study was to explore the particle distribution of EVs in retinal neovascular diseases, including age-related macular degeneration, polypoidal choroidal vasculopathy, and central retinal vein occlusion. The aqueous humor was harvested from 20 patients with different retinal neovascular diseases and six patients with cataracts as the control group. The particle distribution was analyzed using nanoparticle tracking analysis (NTA) and transmitting electron microscopy (TEM). The results revealed that the disease groups had large amounts of EVs and their subtypes compared to the control group. After isolating exosomes, a higher expression of CD81⁺ exosomes was shown in the disease groups using flow cytometry. The exosomes were then further classified into three subtypes of exomeres, small exosomes, and large exosomes, and their amounts were shown to differ depending on the disease type. To the best of our knowledge, this is the first study to elucidate the dynamics of EVs in retinal neovascular diseases using clinical cases. Our findings demonstrated the possible functionality of microvesicles and exosomes, indicating the potential of exosomes in the diagnosis and therapy of retinal neovascular diseases.

【 授权许可】

Unknown