Repeatability of a Commercially Available ELISA Test for Determining the Herd-Level Salmonella enterica subsp. enterica Serovar Dublin Status in Dairy Herds Using Bulk Milk

Frontiers in Veterinary Science

Isabelle Morin²  Marie-Hélène Castonguay²  Khalie Mahamad Amine²  Julie Giguère²  Maryse Michèle Um⁴  Simon Dufour⁴ 

[1] Department of Pathology and Microbiology, Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, QC, Canada;Lactanet, Sainte-Anne-de-Bellevue, QC, Canada;Op+Lait, Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, QC, Canada;Research Group in Epidemiology of Zoonoses and Public Health, Faculty of Veterinary Medicine, University of Montreal, Saint-Hyacinthe, QC, Canada;

关键词: Salmonella Dublin;    ELISA;    bulk milk;    repeatability;    freezing;   

DOI  :  10.3389/fvets.2020.00401

来源: DOAJ

【 摘 要 】

An Enzyme-Linked Immunosorbent Assay (ELISA) is currently available for detection of antibodies against Salmonella Dublin in bovine milk. However, when used in a surveillance program, samples may undergo various storage conditions. The objective of this study was to estimate the repeatability of an ELISA test when used on fresh and frozen samples. Each of 845 bulk milk collected samples was subdivided into 3 aliquots and analyzed using PrioCHECK™ Salmonella Ab Bovine Dublin. ELISA percent positivity results (PP%) were compared between aliquots submitted to the initial analysis and a second analysis conducted 24 h later. The third aliquots were either preserved for 13–14 days (n = 413) or 25–28 days (n = 432) at −20°C prior to analysis and results were compared to the initial analysis. There was excellent concordance between the two initial values and with values obtained after 13–14 and 25–28 days-freezing. The corresponding concordance correlation coefficients were 0.96, 0.97, and 0.94, respectively. Bland-Altman plots showed differences of PP% of 0.1 percentage points on average between the initial and second fresh samples. Freezing for 13–14 and 25–28 days led to overestimation of the initial values by 0.1, and 0.4 percentage points, respectively. Regarding the classification of samples, greater disagreement was observed between 25 and 28 days-frozen and initial samples when using the cut-off 15% (kappa = 0.76) compared to 35% (kappa = 0.90). Our study showed that PrioCHECK™ has good repeatability and that frozen bulk milk samples could generate reliable results. However, the larger variability at lower PP% should be considered when setting up a threshold.

【 授权许可】

Unknown