Genetic Influence on the Peripheral Differentiation Signature of Vδ2+ γδ and CD4+ αβ T Cells in Adults

Cells

LisbethAagaard Larsen¹  Kaare Christensen¹  Kilian Wistuba-Hamprecht²  Svenja Wingerter²  Nicola Beucke²  Karin Hähnel³  Graham Pawelec⁴ 

[1] Danish Twin Register, University of Southern Denmark, 5000 Odense C, Denmark;Department of Dermatology, University Medical Center, 72072 Tübingen, Germany;Department of Internal Medicine II, University Hospital Tübingen, 72076 Tübingen, Germany;Interfaculty Institute for Cell Biology, Department of Immunology, University of Tübingen, 72076 Tübingen, Germany;

关键词: γδ T cell differentiation;    genetic impact;    CMV;    flow cytometry;    immunomonitoring;   

DOI  :  10.3390/cells10020373

来源: DOAJ

【 摘 要 】

Adaptive as well as innate immune traits are variously affected by environmental and genetic influences, but little is known about the impact of genetics on the diversity, differentiation and functionality of γδ T cells in humans. Here, we analyzed a cohort of 95 middle-aged twins from the Danish Twin Registry. The differentiation status of peripheral αβ and γδ T cells was assessed by flow cytometry based on the surface expression of CD27, CD28 and CD45RA. Our data confirm the established associations of latent cytomegalovirus (CMV) infection with an accumulation of late differentiated memory T cells in the αβ compartment as well as in the Vδ1+ γδ T cell subset. A comparison of differentiation phenotypes of γδ and αβ T cells that were not affected by CMV seropositivity identified a significant correlation of early differentiated (ED) Vδ2+ and intermediate differentiated (ID) CD4+ T cells in monozygotic (MZ), but not in dizygotic (DZ) co-twins. Thus, our data suggest a genetic influence on the differentiation of γδ and αß T cell subsets.

【 授权许可】

Unknown