In Vivo Clonal Analysis Reveals Development Heterogeneity of Oligodendrocyte Precursor Cells Derived from Distinct Germinal Zones

Advanced Science

Rui Liu¹  Wenhong Jiang¹  Chong Liu¹  Peng Guo²  Yinhang Jia³  Ruiliang Bai³ 

[1] Department of Neurobiology and Department of Neurosurgery of Second Affiliated Hospital, Zhejiang University School of Medicine Hangzhou Zhejiang 310058 P.R. China;Department of Pathology and Pathophysiology Zhejiang University School of Medicine Hangzhou Zhejiang 310058 P.R. China;Department of Physical Medicine and Rehabilitation of The Affiliated Sir Run Shumen Shaw Hospital Interdisciplinary Institute of Neuroscience and Technology Zhejiang University School of Medicine Hangzhou Zhejiang 310029 P.R. China;

关键词: developmental origin;    heterogeneity;    homeostasis;    in vivo clonal analysis;    NF1;    oligodendrocyte precursor cell;   

DOI  :  10.1002/advs.202102274

来源: DOAJ

【 摘 要 】

Abstract Mounting evidence supports that oligodendrocyte precursor cells (OPCs) play important roles in maintaining the integrity of normal brains, and that their dysfunction is the etiology of numerous severe neurological diseases. OPCs exhibit diverse heterogeneity in the adult brain, and distinct germinal zones of the embryonic brain contribute to OPC genesis. However, it remains obscure whether developmental origins shape OPC heterogeneity in the adult brain. Here, an in vivo clonal analysis approach is developed to address this. By combining OPC‐specific transgenes, in utero electroporation, and the PiggyBac transposon system, the lineages of individual neonatal OPCs derived from either dorsal or ventral embryonic germinal zones are traced, and the landscape of their trajectories is comprehensively described throughout development. Surprisingly, despite behaving indistinguishably in the brain before weaning, dorsally derived OPCs continuously expand throughout life, but ventrally derived OPCs eventually diminish. Importantly, clonal analysis supports the existence of an intrinsic cellular “clock” to control OPC expansion. Moreover, knockout of NF1 could circumvent the distinction of ventrally derived OPCs in the adult brain. Together, this work shows the importance of in vivo clonal analysis in studying stem/progenitor cell heterogeneity, and reveals that developmental origins play a role in determining OPC fate.

【 授权许可】

Unknown