Lemon exosome-like nanoparticles enhance stress survival of gut bacteria by RNase P-mediated specific tRNA decay

iScience

Liqing He¹  Kumaran Sundaram²  Jingyao Mu²  Juw Won Park²  Lifeng Zhang²  Yun Teng²  Shuangqin Zhang²  Xiang Zhang²  Xiangcheng Zhang³  Shao-yu Chen⁴  Mohammed Sayed⁵  Michael L. Merchant⁵  Chao Lei⁵  Wenke Feng⁵  Jun Yan⁵  Mukesh K. Sriwastva⁵  Huang-Ge Zhang⁵  Anil Kumar⁵  Fangyi Xu⁶ 

[1] Department of Medicine, University of Louisville, Louisville, KY 40202, USA;Immunology, University of Louisville, CTRB 309 505 Hancock Street, Louisville, KY 40202, USA;Department of Computer Engineering and Computer Science, University of Louisville, Louisville, KY 40202, USA;Department of ICU, the Affiliated Huaian NO.1 People's Hospital of Nanjing Medical University, Huaian, Jiangsu 223300, China;;James Graham Brown Cancer Center, Department of Microbiology &Kidney Disease Program and Clinical Proteomics Center, University of Louisville, Louisville, KY, USA;

关键词: Nanoparticles;    Molecular biology;    Microbiology;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Diet and bile play critical roles in shaping gut microbiota, but the molecular mechanism underlying interplay with intestinal microbiota is unclear. Here, we showed that lemon-derived exosome-like nanoparticles (LELNs) enhance lactobacilli toleration to bile. To decipher the mechanism, we used Lactobacillus rhamnosus GG (LGG) as proof of concept to show that LELNs enhance LGG bile resistance via limiting production of Msp1 and Msp3, resulting in decrease of bile accessibility to cell membrane. Furthermore, we found that decline of Msps protein levels was regulated through specific tRNAserUCC and tRNAserUCG decay. We identified RNase P, an essential housekeeping endonuclease, being responsible for LELNs-induced tRNAserUCC and tRNAserUCG decay. We further identified galacturonic acid-enriched pectin-type polysaccharide as the active factor in LELNs to increase bile resistance and downregulate tRNAserUCC and tRNAserUCG level in the LGG. Our study demonstrates a tRNA-based gene expression regulation mechanism among lactobacilli to increase bile resistance.

【 授权许可】

Unknown