First record of natural infection by Angiostrongylus cantonensis (Nematoda: Metastrongyloidea) in Belocaulus willibaldoi and Rattus norvegicus in an urban area of São Paulo city, SP, Brazil

Heliyon

Leyva Cecilia Vieira de Melo¹  Ricardo Gava²  Pedro Luiz Silva Pinto²  Vera Lúcia Pereira-Chioccola³  Dan Jessé Gonçalves da Mota⁴ 

[1] Corresponding author.;Laboratório de Biologia Molecular de Parasitas e Fungos, Instituto Adolfo Lutz, Avenida Doutor Arnaldo, 355 - 8º andar- CEP: 01246-000, São Paulo, SP, Brazil;Núcleo de Enteroparasitas, Instituto Adolfo Lutz, Avenida Doutor Arnaldo, 355- 8º andar- CEP: 01246-000, São Paulo, SP, Brazil;Secretaria Municipal da Saúde de São Paulo- (UVIS Parelheiros) - Departamento de Vigilância Sanitária, Rua Cristina Schunck Klein, 23, CEP: 04890-350, São Paulo, SP, Brazil;

关键词: Microbiology;    Molecular biology;    Zoology;    Angiostrongyliasis;    Angiostrongylus cantonensis;    Belocaulus willibaldoi;   

DOI  :  

来源: DOAJ

【 摘 要 】

Angiostrongylus cantonensis, a rat lungworm, is one of the leading causes of eosinophilic meningitis in humans. Infection in humans occurs by the ingestion of intermediate hosts, undercooked paratenic hosts or contaminated vegetables and fruits by mucus from infected molluscs. This zoonosis is widespread in tropical and subtropical areas of Southeast Asia, it has also been reported in the Pacific Islands as well as in other regions of Americas. In Brazil, human cases of angiostrongyliasis have been reported since 2007 in Southeast, Northeast, and South regions. In January 2011, we collected a batch of 30 Belocaulus willibaldoi slides in a neighborhood of São Paulo city (Parque Fernanda). Six of them were used for identifying species, and the others (24) were used in parasitological tests through digestion in peptic solution and then larvae isolation by the Rugai method. A total of 250 larvae were obtained and they had morphological traits of Angiostrongylus spp. Later, four Golden hamsters (Mesocricetus auratus) were infected with 38 larvae that allowed the recovery of young worms from the brain and lungs of rodents on the 21st and 30th day of infection. In this same neighborhood we captured rodents (Rattus norvegicus) that, after necropsy led us to recovery of 22 adult worms in the pulmonary arteries (14 males and 8 females) in May 2011. The larvae and worms obtained from natural infection were evaluated by morphological and morphometric parameters, as well as biological behavior patterns and molecular profile. All methodologies identified the parasite as Angiostrongylus cantonensis. In this way, we report for the first time, the natural infection by A. cantonensis in intermediate (B. willibaldoi) and definitive (R. norvegicus) hosts in a new urban region of Brazil.

【 授权许可】

Unknown