Structural and functional characterization of hemp seed (Cannabis sativa L.) protein-derived antioxidant and antihypertensive peptides

Journal of Functional Foods

Sunday Malomo¹  Jianping Wu²  Rotimi E. Aluko²  Rong He³  Abraham T. Girgih³  Marina Offengenden³ 

[1] College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210046, People’s Republic of China;Department of Agricultural, Food and Nutritional Science, University of Alberta, 4-10 Ag/For Centre, Edmonton, AB T6G 2P5, Canada;Department of Human Nutritional Sciences, The Richardson Centre for Functional Foods and Nutraceuticals, University of Manitoba, Winnipeg, MB R3T 2N2, Canada;

关键词: Hemp seed;    Angiotensin converting enzyme;    Renin;    Antihypertensive peptides;    Antioxidant peptides;    Spontaneously hypertensive rats;   

DOI  :  

来源: DOAJ

【 摘 要 】

The aim of this study was to identify and test efficacy of antioxidant and antihypertensive peptides present in hemp seed protein hydrolysate (HPH), which was produced through simulated gastrointestinal tract digestion of hemp seed proteins. Consecutive fractionation of HPH by reverse-phase HPLC followed by tandem mass spectrometry analysis of active peaks led to identification of 23 short-chain (⩽5 amino acids) peptides. At 0.5 mg/mL, Trp-Val-Tyr-Tyr (WVYY) and Pro-Ser-Leu-Pro-Ala (PSLPA) were the most active antioxidant peptides with 67% and 58% DPPH scavenging and metal chelation activity of 94% and 96%, respectively. WVYY and PSLPA showed maximum systolic blood pressure (SBP) reduction in spontaneously hypertensive rats by 34 (2 h) and 40 mmHg (4 h), respectively, after oral administration of 30 mg/kg body weight dose. Trp-Tyr-Thr (WYT) at 2 h, Ser-Val-Tyr-Thr (SVYT) at 6 h, and Ile-Pro-Ala-Gly-Val (IPAGV) at 4 h, each at 30 mg/kg body weight dose had maximum SBP reductions of 13, 24, and 36 mmHg, respectively.

【 授权许可】

Unknown