Analysis of cannabinoids by liquid chromatography-mass spectrometry in milk, liver and hemp seed to ensure food safety

卷:228

Escriva, Ursula ; Jesus Andres-Costa, Maria ; Andreu, Vicente ; Pico, Yolanda

Univ Valencia

关键词: Cannabinoids;    LC-MS/MS;    Solid-phase extraction;    Food safety;    Hemp seed;    Food of animal origin;   

DOI  :  10.1016/j.foodchem.2017.01.128

学科分类：食品科学和技术

【 摘 要 】

A method for determining cannabinoids, Delta 9-tetrahidrocannabinol (THC), 11-nor-9-carboxy-Delta(9)-THC (THC-COOH) and 11-hidroxy-Delta(9)-THC (THC-OH) in milk, liver and hemp seeds based on liquid chromatography tandem mass spectrometry has been optimized and validated. Analytes were extracted with methanol and the extracts cleaned-up by solid-phase extraction using Oasis HLB (60 mg). The developed method was validated according to the Commission Decision 2002/657/EC. The decision limit (act) and detection capability (CC beta) ranged from 3.10-10.5 ng g(-1) and 3.52-11.5 ng g(-1), the recoveries were 76-118% and matrix effect ranged from -17.8% to 19.9% in the three matrices studied. The method was applied to food samples obtaining positive results for THC in hemp seeds (average 0.82 mu g g(-1)) and three brands of junior formula milk at concentrations from 4.76 to 56.11 ng g(-1). The developed method was suitable achieving identification and quantification of cannabinoids in food matrices. (C) 2017 Elsevier Ltd. All rights reserved.